Capital HPLC - Optimal HPLC Range

Optimal is more than just another new generation HPLC packing material. Optimal is manufactured using an entirely new process based on well established, tried and tested silica production techniques coupled with new refining technologies which ultimately leads to a superior material combining the advantages of both the new and traditional approaches to silica manufacturing.

Specification
The fact that high purity Optimal is manufactured using well established silica engineering techniques means that a wide range of specifications are possible. As a result, we can supply Optimal in pore sizes ranging from 60‰ to 1000‰ with the most popular being 120‰. Moreover, Optimal is also available in particle sizes ranging from 3µm up to preparative grade sizes.

Shape	Spherical
Phases	ODS-H, ODS-L, C8 and Silica
Particle Diameter	3µm, 5µm and larger
Carbon Loading	ODS-H (18%), ODS-L (15%)
Pore Diameter	120‰, 300Å, 500Å and 1000‰
Surface Area	320m²g^-1. (120‰ material)
Typical Efficiency	75,000 plates/m

Optimal ODS-H
Optimal ODS-H is the most generally applicable of the Optimal phases and can be used for both specialist applications involving acid and basic compounds or mixtures of both. Optimal ODS-H is also particularly well suited to general purpose method development.

	The absence of active silanols in Optimal is illustrated by this standard test mixture which includes both pyridine and phenol. The lack of peak tailing clearly demonstrates the absence of metallic impurities. Column: Optimal ODS-H 5µm Dimensions: 15cm x 4.6mm Mobile Phase: 40:60 Acetonitrile:Water Temperature: 40ƒC Flow Rate: 1ml/min Detection: UV at 254nm 1. Pyridine, 2. Phenol
	Neutral silanol groups can still occasionally cause problems with certain classes of compound. Theobromine, theophylline and caffeine are particularly prone to this effect. In Optimal this effect is elimated by full end capping. Column: Optimal ODS-H 5µm Dimensions: 15cm x 4.6mm Mobile Phase: 25:75 Methanol:Water Temperature: 30ƒC Flow Rate: 1ml/min Detection: UV at 254nm 1. Theobromine, 2. Theophylline 3. Caffeine, 4. Phenol
Many compounds such as polyphenols interact irreversibly with the active sites on silica based reversed phase materials. In the case of Optimal ODS-H this problem does not occur, which makes it possible to analyse problematic compounds such as in the example below.
	Column: Optimal ODS-H 5µm Dimensions: 15cm x 4.6mm Mobile Phase: THF:Water:Formic Acid (35:60:5) Temperature: 40ƒC Flow Rate: 0.65Sl/min Detection: UV at 254nm

Optimal ODS-L
Optimal ODS-L has a slightly lower carbon loading (15%) than the ODS-H and is useful for the analysis of a wide range of hydrophilic compounds which are normally not retained by other C18 based packing material.

One particular advantage is that even pure water can be used as the eluent without the need for any organic modifier. Highly polar samples only soluble in water may be separated by this packing material, e.g. sugars and other saccharides (see example below). Optimal ODS-L is also as useful for analyzing hydrophobic samples which are currently chromatographed on competitor C8 materials. Like ODS-H, this packing material is also fully end-capped.

Optimal ODS-L is also based on the same high purity silica as the Optimal ODS-H material which ensures the high degree of inertness and lack of peak tailing.

Column: Optimal ODS-L 5µm
Dimensions: 15cm x 4.6mm
Mobile Phase: 100% Water (HPLC Grade)
Temperature: 40ƒC
Flow Rate: 1ml/min
Detection: Refractive Index

1. Sucrose
2. Kestose
3. Fructotriose
4. Fructotetraose

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